南湖新聞網訊（通訊員 鄧波）近日，我校yl7703永利農業生物質增值利用技術與裝備團隊袁巧霞教授課題組在環境學科領域知名國際學術期刊Water Research上發表了題為“Biotransformation of Pb and As from sewage sludge and food waste by black soldier fly larvae: migration mechanism of bacterial community and metalloregulatory protein scales”的研究論文。該研究借助16s rRNA和宏基因組測序技術，揭示了黑水虻無害化處理污水活性污泥過程中蟲體腸道微生物群落及功能基因對重金屬Pb和As遷移轉化的調控機制，闡明了Pb、As重金屬在蟲體、蟲沙中的賦存形态、演化規律及遷移轉化路徑，該研究成果為黑水虻無害化、資源化轉化處理活性污泥提供了科學依據。
我國每年各類污水活性污泥年産生量超7000萬噸，尤其是富含Pb、As等相關重金屬，不僅環境風險高，而且處理難度大。黑水虻是一種生長速度快、重金屬耐受性高、有機廢棄物轉化能力強的食腐昆蟲，因其獨特的生物學特性，已被成功用于污水活性污泥的無害化處理與資源化利用。但截至目前，污水活性污泥黑水虻無害化處理技術還未能大規模推廣應用，其中關鍵性制約因素是黑水虻轉化處理活性污泥過程中相關重金屬的遷移轉化路徑及其演變鈍化機制有待進一步明确。
針對這一關鍵問題，袁巧霞教授團隊聚焦Pb和As兩種典型的活性污泥重金屬，設計了黑水虻重金屬梯度阈值養殖試驗，并借助16s rRNA和宏基因組測序技術，深入研究了Pb、As重金屬在轉化過程中的形态轉化和遷移路徑，重點解析了成熟幼蟲的腸道微生物群落和金屬調控蛋白的作用規律與調控機制。結果表明，黑水虻轉化降低了重金屬的遷移性，蟲沙中的鉛和砷主要是殘渣态（31-51%），同時幼蟲還可以将32-48%的鉛和砷富集在體内。16s rRNA分析表明鉛和砷脅迫降低了幼蟲腸道中Firmicutes, Romboutsia和Lactobacillus的豐度（6.3-92.0%）。宏基因組測序發現在金屬反應轉錄因子CadC的的調節下，鉛脅迫刺激P型ATP酶ZntA和底物結合蛋白EfeO的表達，從而實現對鉛的結合、轉運和排洩。幼蟲腸道中多種功能蛋白協同調節As (III)的遷移，ArsD 是一種金屬伴侶，可将 As (III) 傳遞給 ArsA，從而提高對 As (III) 的親和力。ArsA 結合催化 ATP 對 As (III) 的吸收，并通過砷外排系統（例如ArsB，ArsJ，ArsR和ArsK）排出砷。
yl7703永利在讀博士研究生鄧波為第一作者，袁巧霞教授和曹紅亮教授為共同通訊作者。本研究得到國家自然科學基金、湖北省科技創新計劃等課題資助。
原文鍊接：https://doi.org/10.1016/j.watres.2024.121405
英文摘要：
The accumulation and transformation of lead (Pb) and arsenic (As) during the digestion of sewage sludge (SS) by black soldier fly larvae (BSFL) remain unclear. In this study, we used 16s rRNA and metagenomic sequencing techniques to investigate the correlation between the microbial community, metalloregulatory proteins (MRPs), and Pb and As migration and transformation. During the 15-day test period, BSFL were able to absorb 34–48% of Pb and 32–45% of As into their body. Changes in bacterial community abundance, upregulation of MRPs, and redundancy analysis (RDA) results confirmed that ZntA, EfeO, CadC, ArsR, ArsB, ArsD, and ArsA play major roles in the adsorption and stabilization of Pb and As, which is mainly due to the high contribution rates of Lactobacillus (48–59%) and Enterococcus (21–23%). Owing to the redox reaction, the regulation of the MRPs, and the change in pH, the Pb and As in the BSFL residue were mainly the residual fraction (F4). The RDA results showed that Lactobacillus and L. koreensis could significantly (P < 0.01) reduce the reducible fraction (F2) and F4 of Pb, whereas Firmicutes and L. fermentum can significantly (P < 0.05) promote the transformation of As to F4, thus realizing the passivation Pb and As. This study contributes to the understanding of Pb and As in SS adsorbed by BSFL and provides important insights into the factors that arise during the BSFL-mediated migration of Pb and As.
審核人：袁巧霞
來源：南湖新聞網http://news.hzau.edu.cn/2024/0306/69109.shtml
